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KMID : 0903619960370050700
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 5 p.700 ~ p.703
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Influence of Cultivar, Light Condition and Pretreatment on Adventitious Shoot Regeneration from Leaves, Internodes and Petioles of Malus domestica Borkh. in Vitro
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Abstract
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The percentage of adventitious shoot regeneration from in vitro leaves, internodes and petioles of ´Gala´ and ´FTRI 2´ was significantly higher than that of the other 7 genotypes. Leaves were better tissue source for shoot regeneration than internodes or petioles. Dark treatment for the initial 2 weeks and 16 hours in bide light for the other 6 weeks was best for adventitious shoot regeneration. The ´Gala´ leaves, taken from mother culture which grown in dark for 1 week before excision, showed higher percentage of adventitious shoot regeneration than those from continuous 16 hours light condition, while ´Fuji´ showed that the percentage of adventitious shoot regeneration was decreased when leaves were taken from dark-treated mother culture. The influence of hormonal condition of mother culture medium on adventitious shoot regeneration of leaf tissues in ´Gala´ and ´Fuji´ was also tested; the highest percentage of adventitious shoot regeneration was obtained from 1.0 ㎎·L^(-1) BA in the both cultivars. IBA 0.1 ㎎·L^(-1) was better for ´Gala´, while 0.5 ㎎·L^(-1) IBA for ´Fuji´. The leaf tissues taken from unrooted mother culture were better for adventitious shoot regeneration than those taken from rooted mother culture.
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